Residue:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
Binding Assay:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
Methylation:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
Western Blot:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
Co-Immunoprecipitation Assay:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
Transfection:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
Immunoprecipitation:Article Title: Arginine Methylation Antagonizes TEAD3-Mediated Repression to Promote Osteogenic Differentiation by Disrupting RUNX2-Sequestrating Condensates.
Article Snippet: .. This residue is located within a conserved GRR motif (residues 54–56) of the TEA DNA binding domain, which is a pre- Advanced Science, 2026 om m ons L icense FIGURE 1 TEAD is arginine methylated during osteogenic differentiation in PDLSCs. (A) Western Blot analysis of MMA during osteogenic differentiation of PDLSCs. (B) Processes to identified arginine methylated protein during osteogenic differentiation. (C) Screen for proteins with identified arginine methylated peptides during osteogenic differentiation. (D) MS analysis of TEAD3 and KRT1. (E) Co-IP was performed by MMA antibody and the pull downed proteins were subjected to Western Blot. (F) Flag-tagged TEAD1-4 were transfected into 293T cells, immunoprecipitated using a Flag-specific antibody, and subsequently analyzed by Western blot. nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202518597, W iley O nline L ibrary on [27/01/2026]. ..
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